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Volume 15 Issue 4
August 2000
    Citation: XIAO Huo-Gen, HU Jin-Sheng, FAN Fu-Zhong. Detection of Papaya Ringspot Virus by Imm uno-capture PCR，Nested-PCR and ELISA-PCR Assays .VIROLOGICA SINICA, 2000, 15(4) : 367-372.
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    Detection of Papaya Ringspot Virus by Imm uno-capture PCR，Nested-PCR and ELISA-PCR Assays

    • 1.

      Lab of Plant 

    • Available online: 05 December 2000
    • Sensitive, rapid, and reliable immuno capture PCR (IC PCR), ELISA PCR and Nested PCR assays were developed for the first time to detect PRV. It was detected from dilutions of 10 -4 , 10 -7 and 10 -14 (equivalent to 0.5 ug, 0.5 ng and 510 -8 ng of papaya leaf tissues) by IC PCR, ELISA PCR and Nested PCR, respectively.
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      Detection of Papaya Ringspot Virus by Imm uno-capture PCR，Nested-PCR and ELISA-PCR Assays

      • 1. Lab of Plant 

      Abstract: Sensitive, rapid, and reliable immuno capture PCR (IC PCR), ELISA PCR and Nested PCR assays were developed for the first time to detect PRV. It was detected from dilutions of 10 -4 , 10 -7 and 10 -14 (equivalent to 0.5 ug, 0.5 ng and 510 -8 ng of papaya leaf tissues) by IC PCR, ELISA PCR and Nested PCR, respectively.

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