Study on the Feline Caticivirus Infected Tiger
Abstract: A virus strain like calicivirus was isolated from organs of tiger with feline kidney (FK) cell. The viruses were nonenveloped, 35~39 nm in diameter, multiplied in cytoplasm and put in crystal order. The viruses multipied easily in feline kidney cell cultures and produced cytopathic effects (CPE). Virus cross neutralization (VN) showed that the virus is closely relation with standard vaccine virus strain. Felines infected with FK cell cultures viruses clinically appeared FCV signs. The RT PCR amplified product around 540 bp which was the same as result of Designed. The PCR products were directly sequenced. The gene sequences were compared with FCV strains extracted from the GenBank database and showed 87% identity. Systematical identification has proved that the viruse isolated is feline calicivirus of tiger. This is the first report of feline calicivirus infected tiger in China.