HSP72．1ike Immunoreactivities in the Brains of M ice Experimentally Infected with Hantavirus

Sueking mice．2～3 day after birth，were intraperitoneally inoculated with 0 O5mL F1M lIl 0f Chen Strain Hantaan virus At different time point after inoculation，the brains were taken，rou— tinely fixed and embedded in paraffin for preparing 5 um serial sections Traditional and confocal im— munochemicaI detection of viral antigens and heat shock protein 70 were performed to explore the cere— hral stress response after viral infection．The results showed that HSP70 imm ur~reactivities could be stably detected in the viral antigen positive neurons，hut not in the viral antigen negative or uninfected control tissues By confocaI microscopic examination．the H趼，70 and viral antigens were colocolized in the neuronal cytoplasm ．Our result，comparable to our previous findings in human tissues and culture cells。indicated that Hantavirus infection can induce the expression of H趼，70 in the infected cells。and HSP70 expresion might he necessary hut not sufficient to keep the cell surviva1．