Citation: CHEN De, PAN Jie-yan, JIA Liqun, CAI Bao-xiang, CHEN Puyan. Sequencing and Homology Analysis for the Nucleocapsid Gene of JS/95/03 Strain of Avian Infectious Bronchitis Virus Isolated from Jiangsu,China .VIROLOGICA SINICA, 2001, 16(1) : 22-27.

Sequencing and Homology Analysis for the Nucleocapsid Gene of JS/95/03 Strain of Avian Infectious Bronchitis Virus Isolated from Jiangsu,China

  • Available online: 05 February 2001
  • Field avian infectious bronchitis virus(IBV)designated as JS/95/o3。which was isolated from Jiangsu province of china,was cultivated in chicken embryo.It’S single strain RNA was extract— ed from purified virus and worked as template of reverse transcription polymexase chain reaction f RT— PCR),a pair of primer designed according to megalign results of published IBV sequences in Genbank was used to amplify the neucleocapsid gene,the RT·PCR product was sequenced directly Sequence analysis revealed that the sequence of JS/95/03 is most homologized with that of M41 strain

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    Sequencing and Homology Analysis for the Nucleocapsid Gene of JS/95/03 Strain of Avian Infectious Bronchitis Virus Isolated from Jiangsu,China

    • 1. 1 Key laboratory of animal disease diagnosticⅡ immun~ogy.Ministry of Agriculture, Nanjing agricultural university.Nanjing 210095,China

    Abstract: Field avian infectious bronchitis virus(IBV)designated as JS/95/o3。which was isolated from Jiangsu province of china,was cultivated in chicken embryo.It’S single strain RNA was extract— ed from purified virus and worked as template of reverse transcription polymexase chain reaction f RT— PCR),a pair of primer designed according to megalign results of published IBV sequences in Genbank was used to amplify the neucleocapsid gene,the RT·PCR product was sequenced directly Sequence analysis revealed that the sequence of JS/95/03 is most homologized with that of M41 strain

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