Citation: LI Li.jun.ZHOU Zhong.ju.XIE Lian-hui, . Cloning of 3’-terminal Part of Sugarcane M osaic Virus RNA and Sequence Comparison of CP Gene Between Chinese Isolate and Other Strains of SCMV .VIROLOGICA SINICA, 2001, 16(1) : 45-50.

Cloning of 3’-terminal Part of Sugarcane M osaic Virus RNA and Sequence Comparison of CP Gene Between Chinese Isolate and Other Strains of SCMV

  • Available online: 05 February 2001
  • ScMV.CA,an isolate of the doraJnant A strain in China continent was propagated on sweet maize and purified.Cloned cDNAs representing more than 0.8kb of the 3’termini were obtained after a reverse transcription and ligation to pUC19 in Sma1 site using an ollgo(dT)primer One clone na med SCMV.CA54 was sequenced and the 1ength of the SCMV—CA54 was 1296 bp.The sequenee covered the 3’untranslated region(3’UTR).coat protein(cP)mad part of the nuclear inclusion b (NIb)genes of the virus.The nucleotide and deduced amino acid~equenee in the CP gene hatweell Chinese isolate SCMV.CA and other isolates or strains of SCMV subgroup were compa red.Results showed that the homology of nucleotide sequence in CP gene between SCMV—CA and other isolates or strains of SCMV subgroup ranged from 63.7% to 77.6% .and amino acid sequence from 64% to 89% .This homology degree lies betwen the range for distinct viruses and tha t betwen related strains of potyviruses,arguing tha t the Chinese isolate might represent a distinct virus.

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    Cloning of 3’-terminal Part of Sugarcane M osaic Virus RNA and Sequence Comparison of CP Gene Between Chinese Isolate and Other Strains of SCMV

    • 1. Institute orPlant Virology ofFujian Agricultural andForestryUniversity.Fuzhou 350002,China

    Abstract: ScMV.CA,an isolate of the doraJnant A strain in China continent was propagated on sweet maize and purified.Cloned cDNAs representing more than 0.8kb of the 3’termini were obtained after a reverse transcription and ligation to pUC19 in Sma1 site using an ollgo(dT)primer One clone na med SCMV.CA54 was sequenced and the 1ength of the SCMV—CA54 was 1296 bp.The sequenee covered the 3’untranslated region(3’UTR).coat protein(cP)mad part of the nuclear inclusion b (NIb)genes of the virus.The nucleotide and deduced amino acid~equenee in the CP gene hatweell Chinese isolate SCMV.CA and other isolates or strains of SCMV subgroup were compa red.Results showed that the homology of nucleotide sequence in CP gene between SCMV—CA and other isolates or strains of SCMV subgroup ranged from 63.7% to 77.6% .and amino acid sequence from 64% to 89% .This homology degree lies betwen the range for distinct viruses and tha t betwen related strains of potyviruses,arguing tha t the Chinese isolate might represent a distinct virus.

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