Cloning and Sequencing of M ovement Protein Gene of Cymbidium M osaic Virus

The viral RNA was extracted from purified cymbidium mosaic virus(CyMV)isolated from Oendrobium orchid cultivated in Hainan island．The gene of the movement protein(MP)was amplifled by mealls of reverse transcription—polymerase chain reaction(RT．PCR)，and cloned into pGEM T easy vector． Sequence analysis showed that the gene fragment contained 3 open reading frames (ORbs)which may be encoding 14 kD、12 kD and 10 kD peptides The nucleotide sequence of the doned gene~agment shared 97．8％ homology with the MP genes of CyMV isolated from orchids cultivated in Hawaii and Sing apore