Enhancing Effect of Thl Cytokine on Imm une Responses of M ice to Recom binant Plasmid Encoding Hepatitis C Virus Core Gene

BALB／c mice were immunized with a recombinant plasmid containing HCV—C gene(pcD NAHCV—C)alone or together with a constructs encoding IL一2 or IL12(pIL一2 or pIL一12)．Anti— HCV—C specific antib0dy in the zer8 was measured with ELISA Spleen cells from experimental and control mice were extracted The mouse myloma SP2／0 cells expressing HCV C antigen served as tar get cells CTL activity was measured by~andard 4h Cr release ossays The results showed that anti— HCV—C antibody A value increased from 1．21 of pcDNAHCV C alone to 2 O9 and 1 73 of pHCV—C coimmunized with PIL一2 or PIL一12 respectively CTL activity to HCV core prote~n was substantially enhanced after coimmunization with the IL 12 expressing plasmid In contrast．the improvement of HCV core—specific CTL activity with an IL一2一pmdudng construct coimmunization was not obvious． whieh could related to the lOW—leve1 expression of IL 2 vector-pDOR DNA vaccine of HCV combined with IL一12 expresing plasmid co uld get better immunizing effects，and【c might represent a gene ther appeudc strategy against the HCV infection of human