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Volume 16 Issue 2
April 2001
    Citation: ZHONG Yan wei, CHENG Jun．SHI Shuang-shuang, el al, . Identification and Expression of Human ScFv Against Surface Antigen of Hepatitis B Virus in E ．coil .VIROLOGICA SINICA, 2001, 16(2) : 105-108.
    shu

    Identification and Expression of Human ScFv Against Surface Antigen of Hepatitis B Virus in E ．coil

    • 1.

      Institute of Infectious Diseasas，The 302 Hospital PLA ，Beifing 100039，China

    • Available online: 05 June 2001
    • Abstract：Using HBsAg antigen purified from }ⅢsAg positive$ar~Ii1 as the coating antigen，the sin— gle—chain variable fragment(ScFv)antibodv to HBsAg has been screened and idemifed from a semi synthetic phage library by phage display technique The SfiI／NotI scFv DNA fragment was harvested from PHENI—ScFv and inserted into pCANTAB5E．and the recombinant plasmid DNA was used to transf0rm competent host XL1 Blue．After induction with IP，rG fnr 20 hrs，the expressed Ⅲ sAg ScFv in the supernantant of XL1一B1He was precipitated with 50％ ammonium sulfated and demonstrat ed the molecular weight is about 28kDa bv SD PAGE The reactivity and spedficity have be n con— firmed by enzyme—linked irnmuno-sorbent assay f ELISA)．The identification and expression of HB— sAg ScFv from E coli were successfully achieyed
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      Identification and Expression of Human ScFv Against Surface Antigen of Hepatitis B Virus in E ．coil

      • 1. Institute of Infectious Diseasas，The 302 Hospital PLA ，Beifing 100039，China

      Abstract: Abstract：Using HBsAg antigen purified from }ⅢsAg positive$ar~Ii1 as the coating antigen，the sin— gle—chain variable fragment(ScFv)antibodv to HBsAg has been screened and idemifed from a semi synthetic phage library by phage display technique The SfiI／NotI scFv DNA fragment was harvested from PHENI—ScFv and inserted into pCANTAB5E．and the recombinant plasmid DNA was used to transf0rm competent host XL1 Blue．After induction with IP，rG fnr 20 hrs，the expressed Ⅲ sAg ScFv in the supernantant of XL1一B1He was precipitated with 50％ ammonium sulfated and demonstrat ed the molecular weight is about 28kDa bv SD PAGE The reactivity and spedficity have be n con— firmed by enzyme—linked irnmuno-sorbent assay f ELISA)．The identification and expression of HB— sAg ScFv from E coli were successfully achieyed

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