Comparison of Producing Dendrolimus punctatus Cytoplasm ic Polyhedrosis Virus in Substitutive Host Insect

Argyrogramma agnata has been selected as a substitutive host insect for producing Den drolimus punctatus Cytoplasmic Polyhedmsis Virus(DpCPV)，In our experiment．it is very suscepti— ble to DpCPV．The DpCPV produced in A．agnata is designated Aa-DpCPV The cytoplasmic poly— hedra body(CPB)．the virion size and the shape of Aa—DpCPV are same as that of its original DpCPV (DpCPV-W 1984) The RNA bands of Aa-DpCPV and DpCPV—W 1984 al1 have 10 RNA segments re— spectively in 3％ PAGE．which molecular weights ranged in size from 2 98×10。to 0 66× 10。Dal— ton．Aa—DpCPV has the saro．e strong toxicity as that of DpCPV-W 1984(from D punctatus)tO D． punctatus(Walker)larva So it cai2 he applied tO the pine caterpillar contro1．The DpCPV yield in A．agnata iS 2．5 x 10 CPB／larva