Isolation and Adaptation of HAV on M EK Cells and Vero Cells

HAV strains W and X were isolated and adapted to Rhesus monkey embryo kidney(MEK) cells from feces of hepatitis A patients,and identified to be HAV with blocking experiment,neutralizing experiment,indirect immunofluorescence (IF),immune electron microscopy(IEM) and RT-PCR.Antigenic titers of strains W and X P7 on MEK cells were 1∶512,1∶1024,respectively and infective titers(logTCID 50 /mL)of that were 8.17 ,8.50 respectively.Only the isolate W was adapted to Vero cells.Antigenic titer of adapted strain W P6 on Vero cells on 21th day was 1∶256 and infective titer(logTCID 50 /mL)was 8.00.