Molecular Characterristics of the Seed Virus 0f Hl S Candidate Vaccine A16 Strain

For further studying on the molecular characteristics of A16 strain,which is the seed virus of HFRS candidate vaccine,the M and S segments of A16 strain were amplified by RT PCR and then sequenced respectively.Sequence assay showed that Msegment of A16 strain contained 3615 nucleotides,encoding 1133 amino acids,and S segment of A16 strain contained 1 770 nucleotides,encoding 429 amino acids.The homology of M segment of A16 strain’s nucleotide sequences and induced amino acid sequences with that of other HTN type virus varied from 75.7% to 90.3% and 85.9% to 97.1%respectively.Namely,the mutation of M segment of A16 strain’s nucleotide sequence and amino acid sequence with that of other HTN type virus varied from 9.7% to 24.3% and 2.9% to 14.1% respectivedly.Like M segment,the homology of S segment of A16 strain’s nucleotide sequences and induced amino acid sequences with that of other HTN type virus varied from 76.0% to 90.0% and 92.1% to 97.7%,and 67.0% to 67.7% and 81.7% to 82.6% with that of SE