Citation: ZHANG Xiao-xia.ZHANG Zhong-xin.DING Qing-quan. Cloning and Sequence Analysis of the iap2 Gene of Helicoverpa armigera Single-nudeocapsid Nudeopolyhedrovinm .VIROLOGICA SINICA, 2001, 16(4) : 338-343.

Cloning and Sequence Analysis of the iap2 Gene of Helicoverpa armigera Single-nudeocapsid Nudeopolyhedrovinm

  • Available online: 05 December 2001
  • An iap 2 gene of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus was identified.It was localized Bam H I F fragment of the genome.Nucleotide sequencing showed the open reading frame was 753nt,encoded 250 amino acids with a predicted size of 29.2kD.A"TATA"box was found-30~-33nt upstream of the translational start codon ATG.There is an early consensus translational motif CAAT,-50~-53nt upstream of the translational start codon ATG.A typical poly(A)signal was identified 46~51nt downstream of the translational stop codon TAA.HaSNPV iap 2 gene encode one typical RING and two BIRs.The alignment of AcMNPV?OpMNPV?LdMNPV?LdMNPV?SeMNPV?DIAP and XIAP indicated that they had consensus BIR motifs and RING zinc finger motif.It was suggested HaNPV IAP2 had a potential anti apoptotic function.

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    Cloning and Sequence Analysis of the iap2 Gene of Helicoverpa armigera Single-nudeocapsid Nudeopolyhedrovinm

    • 1. Wuhan institute of Virology, Academy of Sicences,Wuhan 430071,china

    Abstract: An iap 2 gene of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus was identified.It was localized Bam H I F fragment of the genome.Nucleotide sequencing showed the open reading frame was 753nt,encoded 250 amino acids with a predicted size of 29.2kD.A"TATA"box was found-30~-33nt upstream of the translational start codon ATG.There is an early consensus translational motif CAAT,-50~-53nt upstream of the translational start codon ATG.A typical poly(A)signal was identified 46~51nt downstream of the translational stop codon TAA.HaSNPV iap 2 gene encode one typical RING and two BIRs.The alignment of AcMNPV?OpMNPV?LdMNPV?LdMNPV?SeMNPV?DIAP and XIAP indicated that they had consensus BIR motifs and RING zinc finger motif.It was suggested HaNPV IAP2 had a potential anti apoptotic function.

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