Cloning and Sequence Analysis of the iap2 Gene of Helicoverpa armigera Single-nudeocapsid Nudeopolyhedrovinm

An iap 2 gene of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus was identified.It was localized Bam H I F fragment of the genome.Nucleotide sequencing showed the open reading frame was 753nt,encoded 250 amino acids with a predicted size of 29.2kD.A"TATA"box was found-30～-33nt upstream of the translational start codon ATG.There is an early consensus translational motif CAAT,-50～-53nt upstream of the translational start codon ATG.A typical poly(A)signal was identified 46～51nt downstream of the translational stop codon TAA.HaSNPV iap 2 gene encode one typical RING and two BIRs.The alignment of AcMNPV?OpMNPV?LdMNPV?LdMNPV?SeMNPV?DIAP and XIAP indicated that they had consensus BIR motifs and RING zinc finger motif.It was suggested HaNPV IAP2 had a potential anti apoptotic function.