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Volume 16 Issue 4
August 2001
    Citation: FANG Ming-gang, CHEN Xin, WANG IJuan-zhong, Just M, HU Zhi-hong. Sequence Analysis of the H／rid m -L Region of the Helicoverpa armigera Single-nudeocapsid Nucleopolyhedrovirus(HaSNPV) .VIROLOGICA SINICA, 2001, 16(4) : 355-360.
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    Sequence Analysis of the H／rid m -L Region of the Helicoverpa armigera Single-nudeocapsid Nucleopolyhedrovirus(HaSNPV)

    • 1.

      中国科学院武汉病研究所无脊椎动物病毒学联合开放实验室 湖北武汉 Lab of Virology Laboratory Wageningen University Bennenhaven11 6709

    • Available online: 05 December 2001
    • In order to investigate the genetic organization of the single nucleocapsid nucleopolyhedrovirus (SNPV) of Helicoverpa armigera (HaSNPV),the Hin d III L fragment of the viral genome was sequenced.The fragment contained five complete open reading frames(ORFs) representing ORF227 (a unique gene),a late expression factor 10 gene ( lef 10),a baculovirus structural protein gene( vp 1 054), Ac 55(a homologue of the AcMNPV ORF55) and Ac 56 (a homologue of the AcMNPV ORF56),respectively.These five genes were identified for the first time in HaSNPV.Alignment of baculovirus LEF10 s indicates that they have conserved leucine zipper regions,and aligament of baculovirus VP1054s indicates that they are highly conserved.
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      Sequence Analysis of the H／rid m -L Region of the Helicoverpa armigera Single-nudeocapsid Nucleopolyhedrovirus(HaSNPV)

      • 1. 中国科学院武汉病研究所无脊椎动物病毒学联合开放实验室 湖北武汉 Lab of Virology Laboratory Wageningen University Bennenhaven11 6709

      Abstract: In order to investigate the genetic organization of the single nucleocapsid nucleopolyhedrovirus (SNPV) of Helicoverpa armigera (HaSNPV),the Hin d III L fragment of the viral genome was sequenced.The fragment contained five complete open reading frames(ORFs) representing ORF227 (a unique gene),a late expression factor 10 gene ( lef 10),a baculovirus structural protein gene( vp 1 054), Ac 55(a homologue of the AcMNPV ORF55) and Ac 56 (a homologue of the AcMNPV ORF56),respectively.These five genes were identified for the first time in HaSNPV.Alignment of baculovirus LEF10 s indicates that they have conserved leucine zipper regions,and aligament of baculovirus VP1054s indicates that they are highly conserved.

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