Serving the Society Since 1986
Advanced Search
Volume 16 Issue 4
August 2001
    Citation: HU Rong, MENG Xiao-Lin, XU Jin, WANG Jian, LIJ Wei. Expression of 2．6kb Enhancin Gene Fragment from Helicoverpa atmigera Granulosis Virus .VIROLOGICA SINICA, 2001, 16(4) : 364-368.
    shu

    Expression of 2．6kb Enhancin Gene Fragment from Helicoverpa atmigera Granulosis Virus

    • 1.

      The 2.6kb fragment of enhancing gene from Helicoverpa armigera granulosis virus was inserted into vector pQE32 and expressed successfully in E.coli M15.The synergy of expression productP 102 on Helicoverpa armigera nuclear polyhedrosis virusHaNPVand Bt against the 2nd instar larvae of H.armigera was studied.The results indicated that the accumulated mortality of the larvae increased 6.25%～27.09% on 168h post infection and the median lethal time decrease at least 12.3h in HaNPV+En treated compared with those in HaNPV treated.The accumulated mortality of the larvae increase 28.18% on 72h postinfection and the median lethal time decreased 12.33h in Bt+En treated compared with that in Bt treated.

    • Available online: 05 December 2001
    • The 2.6kb fragment of enhancing gene from Helicoverpa armigera granulosis virus was inserted into vector pQE32 and expressed successfully in E.coli M15.The synergy of expression product(P 102 ) on Helicoverpa armigera nuclear polyhedrosis virus(HaNPV)and Bt against the 2nd instar larvae of H.armigera was studied.The results indicated that the accumulated mortality of the larvae increased 6.25%～27.09% on 168h post infection and the median lethal time decrease at least 12.3h in HaNPV+En treated compared with those in HaNPV treated.The accumulated mortality of the larvae increase 28.18% on 72h postinfection and the median lethal time decreased 12.33h in Bt+En treated compared with that in Bt treated.
    • 加载中
    • 加载中
    PDF

    Article Metrics

    Article views(4244) PDF downloads(985) Cited by()

    Related
    Proportional views

      Expression of 2．6kb Enhancin Gene Fragment from Helicoverpa atmigera Granulosis Virus

      • 1. The 2.6kb fragment of enhancing gene from Helicoverpa armigera granulosis virus was inserted into vector pQE32 and expressed successfully in E.coli M15.The synergy of expression productP 102 on Helicoverpa armigera nuclear polyhedrosis virusHaNPVand Bt against the 2nd instar larvae of H.armigera was studied.The results indicated that the accumulated mortality of the larvae increased 6.25%～27.09% on 168h post infection and the median lethal time decrease at least 12.3h in HaNPV+En treated compared with those in HaNPV treated.The accumulated mortality of the larvae increase 28.18% on 72h postinfection and the median lethal time decreased 12.33h in Bt+En treated compared with that in Bt treated.

      Abstract: The 2.6kb fragment of enhancing gene from Helicoverpa armigera granulosis virus was inserted into vector pQE32 and expressed successfully in E.coli M15.The synergy of expression product(P 102 ) on Helicoverpa armigera nuclear polyhedrosis virus(HaNPV)and Bt against the 2nd instar larvae of H.armigera was studied.The results indicated that the accumulated mortality of the larvae increased 6.25%～27.09% on 168h post infection and the median lethal time decrease at least 12.3h in HaNPV+En treated compared with those in HaNPV treated.The accumulated mortality of the larvae increase 28.18% on 72h postinfection and the median lethal time decreased 12.33h in Bt+En treated compared with that in Bt treated.

        HTML

      Relative (20)

      目录

      This journal is a member of, and subscribes to the principles of, the Committee on Publication Ethics (COPE)

      鄂ICP备05001977号

         

      鄂公网安备 42010602003674号

      Copyright © 2019 Virologica Sinica. All rights reserved

      /

      DownLoad:  Full-Size Img  PowerPoint
      Return
      Return