PAN Lei, BAI Xue—fan, HUANG Chang—xing, LI Guang-yu．CHEN Hong—mei, W EI San—hua．YANG W ei—song, .Construction of HV S and 5’Terminal of S Gene Segment Eukarytic Expression Vectors and Transfection into Vero Cells .VIROLOGICA SINICA, 2002, 17(1): 47-50.
Citation:
PAN Lei, BAI Xue—fan, HUANG Chang—xing, LI Guang-yu．CHEN Hong—mei, W EI San—hua．YANG W ei—song, .
Construction of HV S and 5’Terminal of S Gene Segment Eukarytic Expression Vectors and Transfection into Vero Cells .VIROLOGICA SINICA, 2002, 17(1)
: 47-50.
Construction of HV S and 5’Terminal of S Gene Segment Eukarytic Expression Vectors and Transfection into Vero Cells
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Department of infectious Disease, Tangdu Hospital,The 4th．Military Medical University．Xi'an 710038,China
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Available online:
15 February 2002
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Abstract
Abstract：In order tO provide some data for study of T cell epitope．HV S and 5’termirmI of S gene segment were inserted into an eukarytic expression vector pcDNA 3．1／V5一His TOPO．The recombi— nant expression plasmids peDNA3 1-S and pcDNA3．1-S—N were constructed．Vero cells were trans— fected in vitro with them．The transient expresion of HV S and 5’terminal of S gene segment in veto cells w∞ detected by；ndirect immunofluoreseence assay(IFA)．
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References
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Proportional views
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