Identification of the Receptor for PDV of Spodoptera Iitura M ultinucleoeapsid Nucleopolyhedrovirus
Abstract: Abstract：This research provides the evidence about the identification of haculovirus receptors present in midgut cell lystes．Rabbit antiserum against purified PDV of SpltM NPV was produced Total midgut eel1 proteins were prepared with SDS lysis buffer with or wi出OUt 2-mere~,ptoethanal(2-ME)．After SDS—Polyacrylarnid gel eleetmphoresis(SDS—PAGE)，the midgut eel1 proteins were tranfered to nitro cellulose membrance．Transferred proteinS were renaturalized overnight with 4％ bovine serum albumin (BSA)．Then with PDV—specific antiserum．a virus overlay protein blot as~y(VOPBA)was performed to identify the protein that binds PDV of SpltMNPV．h was shown that PDV of SpltMNPV bound c0 three molecules of approximately 40kD，73kDand 85kD in midgut cel1 lysates．
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