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Volume 17 Issue 2
April 2002
    Citation: LI Zhi, YANG Pin, LIU Hui, LI Wen—xin. Constraction of Replication—defective Hum an Foamy Virus Vector Directing Expression of Foreign Genes .VIROLOGICA SINICA, 2002, 17(2) : 114-118.
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    Constraction of Replication—defective Hum an Foamy Virus Vector Directing Expression of Foreign Genes

    • 1.

      College of life sciences．Wuhan University．Wuhan 430072．China

    • Available online: 05 April 2002
    • A replication—defective vector(pGPSN1一GFP)base on an infections molecular clone of HFV (pHSRV13)was constructed by deletion and replacement of the en’u and bet genes with exprssion cas— setts for neomycin—resistance and EGFP．Vector stocks weir,prodaced by cationic lipid reagents co — transfection of BHK一21 cells with pGPSNI—GFP and helper plasmid P△ GP These vectors are able to tra~sfer foreign genes into several vertebrate cells and steadily express them ．
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      Constraction of Replication—defective Hum an Foamy Virus Vector Directing Expression of Foreign Genes

      • 1. College of life sciences．Wuhan University．Wuhan 430072．China

      Abstract: A replication—defective vector(pGPSN1一GFP)base on an infections molecular clone of HFV (pHSRV13)was constructed by deletion and replacement of the en’u and bet genes with exprssion cas— setts for neomycin—resistance and EGFP．Vector stocks weir,prodaced by cationic lipid reagents co — transfection of BHK一21 cells with pGPSNI—GFP and helper plasmid P△ GP These vectors are able to tra~sfer foreign genes into several vertebrate cells and steadily express them ．

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