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Volume 17 Issue 2
April 2002
    Citation: FENG Zong—di, CHEN Xin—wen, Just M ．Vlak, HU Zhi—hong. Sequence Analysis of the Xba I-I Region of the Single Nucleocapsid Nllcle0polyhedr0Virus of Helicoverpa armigera .VIROLOGICA SINICA, 2002, 17(2) : 125-131.
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    Sequence Analysis of the Xba I-I Region of the Single Nucleocapsid Nllcle0polyhedr0Virus of Helicoverpa armigera

    • 1.

      The Xba I-I fragment located at 18.4～22.8 m.u. of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus genome was sequenced. The fragment contains eight complete ORFs, including putative ubiquitin, 39K/pp31, lef-11, Lsel25, orf507, orf1080, Ac34,Ac38 and a partial orf which potentially encodes C-terminal of P47. orf507 and orf1080 are two putative unique genes of HaSNPV, both contain an early-expression gene motif CAGT and a TATA box in upstream sequences. The ubiquitin gene of HaSNPV shows high identity with baculoviruses and human or yeast ubiquitins. The 39K/pp31 promotor of HaSNPV does not contain late promotor motifs, which exists in other so far identified baculoviral homologues, suggesting it probably has different transcriptional pattern.

    • Available online: 05 April 2002
    • The Xba I-I fragment located at 18.4～22.8 m.u. of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus genome was sequenced. The fragment contains eight complete ORFs, including putative ubiquitin, 39K/pp31, lef-11, Lsel25, orf507, orf1080, Ac34,Ac38 and a partial orf which potentially encodes C-terminal of P47. orf507 and orf1080 are two putative unique genes of HaSNPV, both contain an early-expression gene motif CAGT and a TATA box in upstream sequences. The ubiquitin gene of HaSNPV shows high identity with baculoviruses and human or yeast ubiquitins. The 39K/pp31 promotor of HaSNPV does not contain late promotor motifs, which exists in other so far identified baculoviral homologues, suggesting it probably has different transcriptional pattern.
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      Sequence Analysis of the Xba I-I Region of the Single Nucleocapsid Nllcle0polyhedr0Virus of Helicoverpa armigera

      • 1. The Xba I-I fragment located at 18.4～22.8 m.u. of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus genome was sequenced. The fragment contains eight complete ORFs, including putative ubiquitin, 39K/pp31, lef-11, Lsel25, orf507, orf1080, Ac34,Ac38 and a partial orf which potentially encodes C-terminal of P47. orf507 and orf1080 are two putative unique genes of HaSNPV, both contain an early-expression gene motif CAGT and a TATA box in upstream sequences. The ubiquitin gene of HaSNPV shows high identity with baculoviruses and human or yeast ubiquitins. The 39K/pp31 promotor of HaSNPV does not contain late promotor motifs, which exists in other so far identified baculoviral homologues, suggesting it probably has different transcriptional pattern.

      Abstract: The Xba I-I fragment located at 18.4～22.8 m.u. of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus genome was sequenced. The fragment contains eight complete ORFs, including putative ubiquitin, 39K/pp31, lef-11, Lsel25, orf507, orf1080, Ac34,Ac38 and a partial orf which potentially encodes C-terminal of P47. orf507 and orf1080 are two putative unique genes of HaSNPV, both contain an early-expression gene motif CAGT and a TATA box in upstream sequences. The ubiquitin gene of HaSNPV shows high identity with baculoviruses and human or yeast ubiquitins. The 39K/pp31 promotor of HaSNPV does not contain late promotor motifs, which exists in other so far identified baculoviral homologues, suggesting it probably has different transcriptional pattern.

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