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Volume 17 Issue 2
April 2002
    Citation: WANG Ying, YU Ze, YAO Han, TAO De, CHEN Qu. Affection of Infection of Autographa californica nucleopolyhedroviruson Sf9 Cell Cycle .VIROLOGICA SINICA, 2002, 17(2) : 132-136.
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    Affection of Infection of Autographa californica nucleopolyhedroviruson Sf9 Cell Cycle

    • 1.

      Institue of Entomology．Central China Normal University，Wuhan 430079，China

    • Available online: 05 April 2002
    • Baculovirus infection resulted in many changes in host cells.The progress of the cell cycle of Spodoptera frgiperda IPLB-Sf21-AE clonal isolate 9 (Sf9) cells was directed by Flow cytometry analysis(FACS).The results showed that the whole time of the cell cycle was about 18 hours and the gapes between every two phases were about 6 hours.At 12-18 hours post AcNPV infection,Sf9 cells were arrested in G\-2/M phase.When the cells in G\-1/S phase synchronized by druge were infected by AcNPV,2/3 of the cells was in G\-2/M phase and 1/3 of the cells in S phase.
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      Affection of Infection of Autographa californica nucleopolyhedroviruson Sf9 Cell Cycle

      • 1. Institue of Entomology．Central China Normal University，Wuhan 430079，China

      Abstract: Baculovirus infection resulted in many changes in host cells.The progress of the cell cycle of Spodoptera frgiperda IPLB-Sf21-AE clonal isolate 9 (Sf9) cells was directed by Flow cytometry analysis(FACS).The results showed that the whole time of the cell cycle was about 18 hours and the gapes between every two phases were about 6 hours.At 12-18 hours post AcNPV infection,Sf9 cells were arrested in G\-2/M phase.When the cells in G\-1/S phase synchronized by druge were infected by AcNPV,2/3 of the cells was in G\-2/M phase and 1/3 of the cells in S phase.

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