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Volume 17 Issue 2
April 2002
    Citation: DAI Ya, CHEN De, PAN Jie, JIANG Yan, LIU Xing, LU Yin, LIU Mei, DING Chan, CHEN Pu, CAI Bao-xiang. Am plification，Cloning and Sequence Analysis of S1 Genes from Infectious Bronchitis Virus Isolates JS／95／03 and SD／97／01 .VIROLOGICA SINICA, 2002, 17(2) : 172-177.
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    Am plification，Cloning and Sequence Analysis of S1 Genes from Infectious Bronchitis Virus Isolates JS／95／03 and SD／97／01

    • 1.

      College of Veterinary Medicine．Nanjing agriculture University，Nanjing 210095-China

    • 2.

      Institute of Agrlculturecie~i~es in Jiangsu Coastal Area，Yancheng 224002．China

    • Available online: 05 April 2002
    • Abstract：The entire S1 genes from nephropathognic strain JS／95／03 and respiratory strain SD／97／01 of infectious bronchitis virus were amplified，cloned and sequenced The nucleotide and deduced amino acid sequences of S1 genes from two isolates and ten reference strains were compared． The results showed that JS／95／03 and SD／97／01 were geneticalty closely related to M41 and H120．respectively． So jt speculated that they were possible variants of raceine strains Two isolates were different in pathogenicity．but they genetically related．Only 24 amino acid differences were found between S1 glycoproteins of two isolates， and 15 of them occurred in the region of the first 130 amino acid residues A amino acid differece Rt the position 1 I6 might he involved in pathDgenicity The corctpa~一 son of the predicted secondary structures of S1 glycoproteins from two isolates revealed that only one or a few amino acid differences were r~ponsible for different antigenicity and structure．
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      Am plification，Cloning and Sequence Analysis of S1 Genes from Infectious Bronchitis Virus Isolates JS／95／03 and SD／97／01

      • 1. College of Veterinary Medicine．Nanjing agriculture University，Nanjing 210095-China
      • 2. Institute of Agrlculturecie~i~es in Jiangsu Coastal Area，Yancheng 224002．China

      Abstract: Abstract：The entire S1 genes from nephropathognic strain JS／95／03 and respiratory strain SD／97／01 of infectious bronchitis virus were amplified，cloned and sequenced The nucleotide and deduced amino acid sequences of S1 genes from two isolates and ten reference strains were compared． The results showed that JS／95／03 and SD／97／01 were geneticalty closely related to M41 and H120．respectively． So jt speculated that they were possible variants of raceine strains Two isolates were different in pathogenicity．but they genetically related．Only 24 amino acid differences were found between S1 glycoproteins of two isolates， and 15 of them occurred in the region of the first 130 amino acid residues A amino acid differece Rt the position 1 I6 might he involved in pathDgenicity The corctpa~一 son of the predicted secondary structures of S1 glycoproteins from two isolates revealed that only one or a few amino acid differences were r~ponsible for different antigenicity and structure．

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