Citation: ZHANG Yu—ping, CHEN Sheng—liang, CHEN Li—qu, Tian xian. Sequencing and Expression of PP71 Gene of Human Cytomegalor Virus .VIROLOGICA SINICA, 2002, 17(2) : 189-192.

Sequencing and Expression of PP71 Gene of Human Cytomegalor Virus

  • Available online: 05 April 2002
  • Abstract:PP7I gene Of the Hut~tan cytomegalor virur(HCMV AD一169)strain W,qS amplified by PCR. After digestion by Barn H I and Hin dⅢ.the fragment was cloned into the high level expre~ion vec— tot pET28a Recombinant plasmid pET28a—PP71 was transfonned into E.coli BI 2l(DE3)and ln— duced by IPTG,high 1evel protein V,’as produced,and the target protein WaS 40% of all proteins.Purifl— eatlo~ recovery rate was high and up to 92 4% of the target protein before purification.which provides scientific basis on research of HCM V pathogenesis and diagnosis.

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    Sequencing and Expression of PP71 Gene of Human Cytomegalor Virus

    • 1. Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430071,China

    Abstract: Abstract:PP7I gene Of the Hut~tan cytomegalor virur(HCMV AD一169)strain W,qS amplified by PCR. After digestion by Barn H I and Hin dⅢ.the fragment was cloned into the high level expre~ion vec— tot pET28a Recombinant plasmid pET28a—PP71 was transfonned into E.coli BI 2l(DE3)and ln— duced by IPTG,high 1evel protein V,’as produced,and the target protein WaS 40% of all proteins.Purifl— eatlo~ recovery rate was high and up to 92 4% of the target protein before purification.which provides scientific basis on research of HCM V pathogenesis and diagnosis.

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