Sequencing and Expression of PP71 Gene of Human Cytomegalor Virus

Abstract：PP7I gene Of the Hut~tan cytomegalor virur(HCMV AD一169)strain W,qS amplified by PCR． After digestion by Barn H I and Hin dⅢ．the fragment was cloned into the high level expre~ion vec— tot pET28a Recombinant plasmid pET28a—PP71 was transfonned into E．coli BI 2l(DE3)and ln— duced by IPTG，high 1evel protein V,’as produced，and the target protein WaS 40％ of all proteins．Purifl— eatlo~ recovery rate was high and up to 92 4％ of the target protein before purification．which provides scientific basis on research of HCM V pathogenesis and diagnosis．