Study on the Endonuelease Site of G1 Region of Hantavirus Genome
Abstract: Typing RT—PCR was used to amplify hantaviruses genome from peripheral blood samples of 34 patients with hemorrhagic fever with renal syndrom e hospitalized in W uhan.The products contained within G 1 gene of M segm ent, w ere digested with H in C II and Sac I respectively, thereby, han— tavirus could be typed into HTN and SEO.The results of RFLP—PCR were highly consistent with typ— ing RT—PCR,and furthermore,RFI P—PCR could quickly screen the variant strain with gene mutation at the restriction site of HiI"l C II or SacI.