Interaction of Adenovirus E 1 B 55kD Oncoproteins with hDaxx
Abstract: Abstract:To question the reason of CO—localization of hDaxx and PM L in PODs disrupted by Aden— ovirus(Ad)E1 B 55一kilodalton oncoproteins(Ad E1 B 55kD),the interaction of Ad E1 B 55kD with hDaxx was analyzed by the yeast two-hybrid assay, and the direct binding of Ad E1 B 55kD with hDaxx was studied by coimm unoprecipitation reaction in vivo or in vitro.The results show that Ad E1B 55kD oncoproteins interact with hDaxx by binding the C-terminus 58 am ino acids of A d2 E1B 55kD or the full length of Adl2 E1B 55kD.Ad2/5 or Adl2 E1B 55kD directly binds hDaxx in vivo or in vitro.