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Volume 17 Issue 3
June 2002
    Citation: WU Xing—quan, W U Zu—j ian, XIE Lian—hui“, I IN Qi—ying. The Clone and Expression of the Coat Protein Gene of Potato Virus S in E ．coli .VIROLOGICA SINICA, 2002, 17(3) : 248-251.
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    The Clone and Expression of the Coat Protein Gene of Potato Virus S in E ．coli

    • 1.

      Plant Virus Institute of F ian Agriculture and Forestry University，Fuzhou 350008，China

    • Available online: 05 June 2002
    • Based on the PVS coat protein gene sequence(885bp)a pair of specific primers were de— signed and the coat protein(CP)gene of potato virus S(PVS)was amplified by RT—PCR．The gene was cloned into pGEX一2T vector and then transformed into E ．coli DH5a．The gene has high homolo— gy(95％ )comparing with other isolate genes．The expression vector p2TSCP was constructed．The coat protein gene was expressed in E ．coli DH5a and a 58kD protein was analysed by SDS—PAGE．
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      The Clone and Expression of the Coat Protein Gene of Potato Virus S in E ．coli

      • 1. Plant Virus Institute of F ian Agriculture and Forestry University，Fuzhou 350008，China

      Abstract: Based on the PVS coat protein gene sequence(885bp)a pair of specific primers were de— signed and the coat protein(CP)gene of potato virus S(PVS)was amplified by RT—PCR．The gene was cloned into pGEX一2T vector and then transformed into E ．coli DH5a．The gene has high homolo— gy(95％ )comparing with other isolate genes．The expression vector p2TSCP was constructed．The coat protein gene was expressed in E ．coli DH5a and a 58kD protein was analysed by SDS—PAGE．

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