Citation: DING Yong, W U, ZHANG W, ZHAO M, TAN Yun. Study on Recombinant HPV DNA Vaccine Inducing Specific Lymphoproliferation .VIROLOGICA SINICA, 2002, 17(4) : 304-307.

Study on Recombinant HPV DNA Vaccine Inducing Specific Lymphoproliferation

  • Available online: 05 November 2002
  • Using molecular cloning technique,HPV16 E7 gene was cloned into eukarytic expression vector.HPV16 E7 DNA vaccine was constructed and Balb/c mice were immunized by intradermal ad— ministration with HPV16 E7 vaccine.After immunization,mice spleen lymphocytes were prepared and restimulated by E7 protein in vitro.Specific lympproliferation were detected by MTT colorimetric as— say.Because detection of specific lymphoproliferation is a simple and efficient way,which can reflect cell—mediated immunity.The study showed that the construction of HPV16 E7 vaccine was correct.It can induce a specific cell—mediated immunization

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    Study on Recombinant HPV DNA Vaccine Inducing Specific Lymphoproliferation

    • 1. 1.Hubei Medical stuff College,Jingzhou 434000,China
    • 2. Medical College,Wuhan University,Wuhan 430071,China

    Abstract: Using molecular cloning technique,HPV16 E7 gene was cloned into eukarytic expression vector.HPV16 E7 DNA vaccine was constructed and Balb/c mice were immunized by intradermal ad— ministration with HPV16 E7 vaccine.After immunization,mice spleen lymphocytes were prepared and restimulated by E7 protein in vitro.Specific lympproliferation were detected by MTT colorimetric as— say.Because detection of specific lymphoproliferation is a simple and efficient way,which can reflect cell—mediated immunity.The study showed that the construction of HPV16 E7 vaccine was correct.It can induce a specific cell—mediated immunization

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