Citation: LI Zhi—guang, YIN Jun, ZHONG Jiang. Expression of Full Length and N Termial Truncated Enhancin from Trochoplusia ni granulovirus in AcM NPV and Analysis of Their Activit .VIROLOGICA SINICA, 2002, 17(4) : 326-330.

Expression of Full Length and N Termial Truncated Enhancin from Trochoplusia ni granulovirus in AcM NPV and Analysis of Their Activit

  • Available online: 05 November 2002
  • Enhancin is a group of baculovirus proteins capable of increasing the infectivity of viruses in insect larvae,as well as the insecticidal effect of other biology control agents. In order to study the structure and function of enhancin from Trichoplusia i gran ulovirus, recombinant baculoviruses were constrcuted to express truncated forms of enhancin with 150, 186 and 250 am ino acids deleted from N terminal of native enhancin,respectively.The truncated enhancins were expressed successfully in Tn一5B1—4 cells. In vitro peritrophic membrane assay showed that all three truncated enhancin lost their mucin degrading ability,while a full length recombinant enhancin was active in the assay.It is concluded that the N terminal amino acids is essential for the mucin degrading activity of enhancin.

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    Expression of Full Length and N Termial Truncated Enhancin from Trochoplusia ni granulovirus in AcM NPV and Analysis of Their Activit

    • 1. Department of Microbiology and Microbial Engineering,School of Life Sciences,Fudan University,Shanghai 200433,China

    Abstract: Enhancin is a group of baculovirus proteins capable of increasing the infectivity of viruses in insect larvae,as well as the insecticidal effect of other biology control agents. In order to study the structure and function of enhancin from Trichoplusia i gran ulovirus, recombinant baculoviruses were constrcuted to express truncated forms of enhancin with 150, 186 and 250 am ino acids deleted from N terminal of native enhancin,respectively.The truncated enhancins were expressed successfully in Tn一5B1—4 cells. In vitro peritrophic membrane assay showed that all three truncated enhancin lost their mucin degrading ability,while a full length recombinant enhancin was active in the assay.It is concluded that the N terminal amino acids is essential for the mucin degrading activity of enhancin.

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