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Volume 17 Issue 4
August 2002
    Citation: WU Dong, W ANG Hua—lin, DENG Fei, CHEN Xin—wen, PENG Hui—yin。HU Zhi—hong, . Molecular Cloning and Expression of the Chitinase Gene from Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus in E.coli .VIROLOGICA SINICA, 2002, 17(4) : 331-335.
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    Molecular Cloning and Expression of the Chitinase Gene from Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus in E.coli

    • 1.

      Joint—lab of Invertebrate Virology，Wuhan Institute of Virology，Chinese Academy of Sciences，Wuhan 430071．China

    • Available online: 05 November 2002
    • The PCR product of the HaSNPV chitinase gene，which was without the N—terminal signal peptide and the C-terminal endoplasmic reticulum location signal，was cloned into a prokaryotic expres— sion vector pProEXHTb．After the induction of IPTG，the chitinase gene was successfully expressed in Eschechia coli DH5a．The expression product shown a molecular weight of 60 kDa，and it covered about 40％ of the total protein in E ．coli．W estern blot analysis using an antibody derived from AcM — NPV ehitinase confirm ed the expression product was a homologue of baculoviral chitinase．
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      Molecular Cloning and Expression of the Chitinase Gene from Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus in E.coli

      • 1. Joint—lab of Invertebrate Virology，Wuhan Institute of Virology，Chinese Academy of Sciences，Wuhan 430071．China

      Abstract: The PCR product of the HaSNPV chitinase gene，which was without the N—terminal signal peptide and the C-terminal endoplasmic reticulum location signal，was cloned into a prokaryotic expres— sion vector pProEXHTb．After the induction of IPTG，the chitinase gene was successfully expressed in Eschechia coli DH5a．The expression product shown a molecular weight of 60 kDa，and it covered about 40％ of the total protein in E ．coli．W estern blot analysis using an antibody derived from AcM — NPV ehitinase confirm ed the expression product was a homologue of baculoviral chitinase．

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