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Volume 17 Issue 4
August 2002
    Citation: WANG Shu—hui 。XIONG Kun, YANG Yi—shu, ZHU Yi—xin, XIA Qiu—yu, CHEN Guo-min, WANG Jin—zhong, CHEN Qi—min, GENG Yun—qi 一, ZENG Yi. The Study on BIV Activity in Human Cells M T -4 .VIROLOGICA SINICA, 2002, 17(4) : 354-357.
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    The Study on BIV Activity in Human Cells M T -4

    • 1.

      1．College ofLife Sciences，Nankai University，Tia， in 300071，China

    • Available online: 05 November 2002
    • Bovine immunodeficiency virus(BIV)is a kind of Lentivirus belonging tO Retrovirus，and up tO now．there is no report about itS infection tO human．In order to check BIV S infection to human cells，we transfected BIVl27 cDAN into human cells MT一4．By RT～PCR，we checked the transcription of BIV S gag gene，and determined the expression of gag or gag-pol gene in M T一4 cell by IFA．Re— verse Transcriptase(RT)assay showed the reverse transcriptase of BIVl27 had been expressed，while cell infection experiment indicated that BIV couldn t replicate in MT一4 cells．
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      The Study on BIV Activity in Human Cells M T -4

      • 1. 1．College ofLife Sciences，Nankai University，Tia， in 300071，China

      Abstract: Bovine immunodeficiency virus(BIV)is a kind of Lentivirus belonging tO Retrovirus，and up tO now．there is no report about itS infection tO human．In order to check BIV S infection to human cells，we transfected BIVl27 cDAN into human cells MT一4．By RT～PCR，we checked the transcription of BIV S gag gene，and determined the expression of gag or gag-pol gene in M T一4 cell by IFA．Re— verse Transcriptase(RT)assay showed the reverse transcriptase of BIVl27 had been expressed，while cell infection experiment indicated that BIV couldn t replicate in MT一4 cells．

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