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Volume 17 Issue 4
August 2002
    Citation: CHENG Zi—qiang, ZHAO Zhen—hua, HAO Yong—qing, ZHAO Xin—li, H ASI Agual. Serology Investigatio and PCR Diagnosis of Avian M yelocytomatosis .VIROLOGICA SINICA, 2002, 17(4) : 371-373.
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    Serology Investigatio and PCR Diagnosis of Avian M yelocytomatosis

    • 1.

      1．Institute ofAnimal Science and Animal Medicine，ShanDong Agriculture University，TaiAn 271018

    • 2.

      Institute of Animal Science and Animal Medicine，Inner Mongolia Agriculture University，Huhhot 010018，China

    • 3.

      Examination Croup of Inner Mongolia Veterinary Station，Huhhot 010020

    • Available online: 05 November 2002
    • We investigated the infection of Avian leukosis virus subgroup J myelocytomatosis usingEI ISA method in Inner Mongolia Region．The result indicated that the meat—type flocks of three dif—ferent farms infection rate'．of ALV—J were 28．12％(9／32)、9．38％(3／32)and 8．33％(10／12)，respec—tively，and all laying chickens infection rate were 0％(0／35，0／10)．We chose 6 chickens of ALV—J an—tibody positive and 13 chickens of ALV—J antibody negative to do PCR test．The result indicated that6 chickens of ALV—J antibody positive were all PCR positive，and 4 chickens were PCR positive among1 3 chickens of AI V—J antibody negative．The result indicated that there were ALV—J virus not only inAI V—J antibody positive chickens but also in some AI V—J antibody negative chickens．
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      Serology Investigatio and PCR Diagnosis of Avian M yelocytomatosis

      • 1. 1．Institute ofAnimal Science and Animal Medicine，ShanDong Agriculture University，TaiAn 271018
      • 2. Institute of Animal Science and Animal Medicine，Inner Mongolia Agriculture University，Huhhot 010018，China
      • 3. Examination Croup of Inner Mongolia Veterinary Station，Huhhot 010020

      Abstract: We investigated the infection of Avian leukosis virus subgroup J myelocytomatosis usingEI ISA method in Inner Mongolia Region．The result indicated that the meat—type flocks of three dif—ferent farms infection rate'．of ALV—J were 28．12％(9／32)、9．38％(3／32)and 8．33％(10／12)，respec—tively，and all laying chickens infection rate were 0％(0／35，0／10)．We chose 6 chickens of ALV—J an—tibody positive and 13 chickens of ALV—J antibody negative to do PCR test．The result indicated that6 chickens of ALV—J antibody positive were all PCR positive，and 4 chickens were PCR positive among1 3 chickens of AI V—J antibody negative．The result indicated that there were ALV—J virus not only inAI V—J antibody positive chickens but also in some AI V—J antibody negative chickens．

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