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Volume 17 Issue 4
August 2002
    Citation: CHENG Kai, WANG Chun—yan, GUO Ya—xin, SHI Zheng—li 。ZHAO Yi—jun, . Measurement of Lysing Cycle and Burst Size of Cyanophage Infecting Filamentous Cyan0bacteria(blue-green algae) .VIROLOGICA SINICA, 2002, 17(4) : 374-376.
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    Measurement of Lysing Cycle and Burst Size of Cyanophage Infecting Filamentous Cyan0bacteria(blue-green algae)

    • 1.

      1．College ofLife Science，Centeral China Normal University，Wuhan 430079，China

    • 2.

      Joint Laboratory of Invertebrate Virology，Wuhan Institute of Virology，the Chinese Academy of Sciences．Wuhan 43007 1．China

    • Available online: 05 November 2002
    • Several methods were t~ted for measurement of lysing cycle and burst size of the cyanophage infecting the ilia— mentous cyanobacterium—Plectonema boryanum IU594．The rm~ts indicated that the lysing curve of host cells was con— sistent with the one-step growth curve of cyanophage，thus it was more convenient tO substitute measuring the lysing curve of host cells for the one—step growth curve measurement of cyanophage．Besides，the burst size increased with the re— ducing cyanophage inoculation．reaching the maxium of 206PFU／Cel by 1 PFU infection．It was suggested that more ex— act burst size of cyanophage could be obtained under 1PFU cyanophage inoculation．
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      Measurement of Lysing Cycle and Burst Size of Cyanophage Infecting Filamentous Cyan0bacteria(blue-green algae)

      • 1. 1．College ofLife Science，Centeral China Normal University，Wuhan 430079，China
      • 2. Joint Laboratory of Invertebrate Virology，Wuhan Institute of Virology，the Chinese Academy of Sciences．Wuhan 43007 1．China

      Abstract: Several methods were t~ted for measurement of lysing cycle and burst size of the cyanophage infecting the ilia— mentous cyanobacterium—Plectonema boryanum IU594．The rm~ts indicated that the lysing curve of host cells was con— sistent with the one-step growth curve of cyanophage，thus it was more convenient tO substitute measuring the lysing curve of host cells for the one—step growth curve measurement of cyanophage．Besides，the burst size increased with the re— ducing cyanophage inoculation．reaching the maxium of 206PFU／Cel by 1 PFU infection．It was suggested that more ex— act burst size of cyanophage could be obtained under 1PFU cyanophage inoculation．

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