Citation: ZHANG Wei, WANG Wei, AI Xiu-lian”, XIE Yu-qing, FEN Huai-rong. Construction of Agrotis segetum granulosis virus cDNA Library .VIROLOGICA SINICA, 2003, 18(1) : 27-30.

Construction of Agrotis segetum granulosis virus cDNA Library

  • Available online: 02 February 2003
  • Abstract:A general cDNA library of Agrotis segetum granulosis virus(ASGV)which was from infected A grotis segetum(As)l arva has been constructed.The first and second cDNA strands were synthesized by AMV reverse transcriptase from mRNA which was isolated from total RNA of infected As larva. So the general cDNA library includes AsGV’S and AS’S, Th en the library of AsGV included 108 1 positive clones were screened by spot hybridization, Th e labeled AsGV genome DNA probe With DIG kit was cut by restriction enzyme of EcoR I and Hind 11. Th e sequence of positnive clones accord with the genome of AsGV,and there are 59 coding sequence which were amplified with synthetic primer of As— GV genome according entirely with the genome of AsGV.

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    Construction of Agrotis segetum granulosis virus cDNA Library

    • 1. Detector ofGene Biotechnology Laboratory,Institute ofMicrobiology xiaj~ gAcademy ofAgricultural Science, Xinjiang Wulumuqi 830000,China

    Abstract: Abstract:A general cDNA library of Agrotis segetum granulosis virus(ASGV)which was from infected A grotis segetum(As)l arva has been constructed.The first and second cDNA strands were synthesized by AMV reverse transcriptase from mRNA which was isolated from total RNA of infected As larva. So the general cDNA library includes AsGV’S and AS’S, Th en the library of AsGV included 108 1 positive clones were screened by spot hybridization, Th e labeled AsGV genome DNA probe With DIG kit was cut by restriction enzyme of EcoR I and Hind 11. Th e sequence of positnive clones accord with the genome of AsGV,and there are 59 coding sequence which were amplified with synthetic primer of As— GV genome according entirely with the genome of AsGV.

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