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Volume 18 Issue 1
February 2003
    Citation: YU Hong-chun, ZHENG Gui-ling, WANG Xiao-yun, LI Guo—xun, ZHAO Kui-jun. Establishment of a cell line from embryos of Mythimna separata and its susceptibility to M sNPV .VIROLOGICA SINICA, 2003, 18(1) : 31-34.
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    Establishment of a cell line from embryos of Mythimna separata and its susceptibility to M sNPV

    • 1.

      1．Northeast Agricultural University,Harbin 150030，China．,2．Hebei Agricultural University,Baoding 071001，China

    • Available online: 02 February 2003
    • Abstract： A new cell line，designated NEAU—Ms一9803 12，was established from 24一hr—old Mythimna separata eggs and was passaged 201 times．The cells were heterogeneous in morphology with spherical and spindle—shaped cells．Th e population doubling time of the cell line aws 47．82h in 120h at 28~C． The cell line was highly susceptible to Mythimna separata nll~leal"polyhedrosis vu’us (MsNPV)．When the cells were inoculated at a multiplicity of infection (m．o．i．)of 0．08TCID50／cell with wild pas— sage MsNPV。the infection rates and occlusion bodies f OB) yield reached a value of 62．10％ ， 33．59PIB／infected cell at 240h postinfection(pi)，respectively
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      Establishment of a cell line from embryos of Mythimna separata and its susceptibility to M sNPV

      • 1. 1．Northeast Agricultural University,Harbin 150030，China．,2．Hebei Agricultural University,Baoding 071001，China

      Abstract: Abstract： A new cell line，designated NEAU—Ms一9803 12，was established from 24一hr—old Mythimna separata eggs and was passaged 201 times．The cells were heterogeneous in morphology with spherical and spindle—shaped cells．Th e population doubling time of the cell line aws 47．82h in 120h at 28~C． The cell line was highly susceptible to Mythimna separata nll~leal"polyhedrosis vu’us (MsNPV)．When the cells were inoculated at a multiplicity of infection (m．o．i．)of 0．08TCID50／cell with wild pas— sage MsNPV。the infection rates and occlusion bodies f OB) yield reached a value of 62．10％ ， 33．59PIB／infected cell at 240h postinfection(pi)，respectively

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