Citation: YUAN Hui-jun, HU Rong-liang”, ZHANG Shou-feng, ZHANG Mao—lin, TU Chang—chun. Characteristics of the Giycoprotein cDNA and Antigenicity of Rabies virus Vaccine Strain SRV9 .VIROLOGICA SINICA, 2003, 18(1) : 63-67.

Characteristics of the Giycoprotein cDNA and Antigenicity of Rabies virus Vaccine Strain SRV9

  • Available online: 02 February 2003
  • 0ral vaccine candidate of Rabies virus strain SRV9 is an intermediate plaque subclone of SAD B 19 and has been used in field with good safety and immunogenicity.A pair of primers was designed according to the published sequence of rabies virus glycoprotein.Reverse transcription—polymerase chain reaction(RT—PCR)was emoloyed to amplify the full cDNAs of the SRV9 and its ancestor SAD B 19.Sequencing result has shown that the RT-PCR product was 1 588bp in length in which a 1 575bp open reading frame was included.It encodes a mature polypeptide composing of 505 amino acids.Compared to SAD B19,base transversions occurred at positions 158,575 and 931,resulting in amino acid mutations at 53, 1 92 and 3 1 1 residues in polypeptide.Compared to the vaccine strain ERA in ues.ten base differences and 7 amino acid alterations were marked.Antigenic index analyses found that the mutation in 192 amino acids resuhed in the form ation of a new potential antigenic site. As the glycoprotein of rabies virus is the only antigen inducing neutralizing antibodies,the alteration of Hi Ain 197 position may be the causes of forming a special plaque and further improvement of its safety.Keeping analyses on amino acids of the glycoprotein of rabies virus is also an important印- proach of surveillance on rabies virus strains

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    Characteristics of the Giycoprotein cDNA and Antigenicity of Rabies virus Vaccine Strain SRV9

    • 1. Military Veterinary Institute,The queenmaster University,Changchun 130062,China

    Abstract: 0ral vaccine candidate of Rabies virus strain SRV9 is an intermediate plaque subclone of SAD B 19 and has been used in field with good safety and immunogenicity.A pair of primers was designed according to the published sequence of rabies virus glycoprotein.Reverse transcription—polymerase chain reaction(RT—PCR)was emoloyed to amplify the full cDNAs of the SRV9 and its ancestor SAD B 19.Sequencing result has shown that the RT-PCR product was 1 588bp in length in which a 1 575bp open reading frame was included.It encodes a mature polypeptide composing of 505 amino acids.Compared to SAD B19,base transversions occurred at positions 158,575 and 931,resulting in amino acid mutations at 53, 1 92 and 3 1 1 residues in polypeptide.Compared to the vaccine strain ERA in ues.ten base differences and 7 amino acid alterations were marked.Antigenic index analyses found that the mutation in 192 amino acids resuhed in the form ation of a new potential antigenic site. As the glycoprotein of rabies virus is the only antigen inducing neutralizing antibodies,the alteration of Hi Ain 197 position may be the causes of forming a special plaque and further improvement of its safety.Keeping analyses on amino acids of the glycoprotein of rabies virus is also an important印- proach of surveillance on rabies virus strains

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