The Interaction of Viral Nucleocapsid Protein with GRP94 and HSP27 in Brains of Suckling M ice Experimentally Infected with Hantaan virus

To study the association of heat-shock protein GRP94 an d HSP27 with Hantaan virus nucleocapsid protein(HTNV—NP)during viral replication，new—bom mice less than 72h of age were experimentally infected with Hantaan virus intracerebrally．Brains of mi ce at the 8th day post-infection were prepared for tissue sections an d extracts．Hantaan virus infection，GRP94，HSP27 expression an d their localization were demonstrated by immunohistochemi cal staining．The possible interaction between HTNV—NP an d GRIX)4．HSP27 was an alyzed by ELISA，immunoprecipitation．HTNV infection led to increased expression of GRIX)4 but not HSP27 at cytoplasma of neurons in brains of infected suckling mice．colocalizationof GRIX)4 with HTNV—NP,an d association of both GRP94 and HSP27 with HTNV—NP．Th e results provide the basis for further research of the roles of HSPs in the viral infection and replication．