Detection of Human cytomegalovirus Infection by PCR-Hybridization-ELISA

A PCR—M PH—ELISA method detecting HCMV infection was established．The PCR products of HCMV DNA were binting—labeled by a 5’-biotinlabeled primer through sample am plification．After the labeled product was mixed with a 5’-digoxin—labeled probe，hybridization was carried out for 2 mi nutes at 90℃ and for 1 minute at 55℃ ．then the hybridized product was captured on microplate wells coated with streptoavidin and detected with a peroxidase—labeled an tibody and TMB substrate． The sensitivity of the method was 2．5×1 04 copies／mL ． and higher than that of conventiona1 PCR and ELISA method．The results did not cross—react with that of HSV1，HSV2，RuV,EBV and ADV DNA． Th e method has potentia1 use for qualitative an d quantitative detection of HCMV infection．