Cloning and Sequencing off gene of Avian paramyxovirus Type I Isolate

Abstract：The f gene was amplified with one—step RT—PCR，cloned and sequenced from three virus isolates(Avian paramyxovirus type I)，which were isolated from diferent avian (chicken and pigeon) inYunnan province．Th e result of the nucleotide sequence an alysis indicated that the homology between the three strains studied was 88．1％-94．9％ ：while the studied strains with refe；rence strains LaSota and F48F-,9 only showed 85．6％ homology．Th e study on the cleavage site amino acid sequence deduced from the nucleotide sequence(residues 1 1 2-1 1 7)showed that the two NDV su~ns matched to all NDV velogenic strains and the Pigeon paramyxovirus(PPMV)I strain matched to PPMV ZQ98—1 strain(one mesogenic strain)，SO the former were velogenic strains and the later was mesogenic strain．Phylogenetic tree constructed with 16 reference strains of 1 662nt(ORF region)of thef gene showed that the two NDV strains should belongs to genotype VII an d the PPM V strain belongs to genotype VI．