Citation: LU Yin-hua, XU Lihua, HUA Xiu-guo, TAN Guo, HUANG Wei-jian, CHEN Desheng, CHEN Puyan. Rapid Detection of Porcine circovirus Type 2 and Porcine reproductive and respiratory syndrome vtrus .VIROLOGICA SINICA, 2003, 18(2) : 184-186.

Rapid Detection of Porcine circovirus Type 2 and Porcine reproductive and respiratory syndrome vtrus

  • Available online: 15 April 2003
  • Abstract:According to the published genome seqflences of Porcine circovirus type 2(PCV2)and Porcine reproductive and respiratory syndrome virus(PRRSV),primers were designed and PCR, RT-PCR were set up for the detection of PCV2 an d PRRS respectively.W ith the established meth ods, 38 clinical samples from the respiratory disease pigs were detected for the presence of PCV2 an d PRRSV.The results demonstrated that 22 sam ples were posirive for PCV2,27 sam ples were positive for PRRSV an d am ong th e above positive sam ples,1 8 sam ples were positive fo r both viruses.The data obtained in the present study indicated that PCV2 an d PRRSV maybe play an important role in the course of the development of respiratory diseases.

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    Rapid Detection of Porcine circovirus Type 2 and Porcine reproductive and respiratory syndrome vtrus

    • 1. 1.Key laboratory of animal disease diagnostic and immunology,Ministry of Agriculture Nanjing agricultural university, Nanjing 210095,China
    • 2. Animal Science Department of Agricultural college,Ningxia University, Hnchuan,750105,China-3.College ofagriculture,Shanghai Jiaotong University,Shanghai,200030,China

    Abstract: Abstract:According to the published genome seqflences of Porcine circovirus type 2(PCV2)and Porcine reproductive and respiratory syndrome virus(PRRSV),primers were designed and PCR, RT-PCR were set up for the detection of PCV2 an d PRRS respectively.W ith the established meth ods, 38 clinical samples from the respiratory disease pigs were detected for the presence of PCV2 an d PRRSV.The results demonstrated that 22 sam ples were posirive for PCV2,27 sam ples were positive for PRRSV an d am ong th e above positive sam ples,1 8 sam ples were positive fo r both viruses.The data obtained in the present study indicated that PCV2 an d PRRSV maybe play an important role in the course of the development of respiratory diseases.

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