Construction of Recombinant Adenovirus Expressing Influenza virus Neuraminidase Gene
Abstract: Abstract:1]he neuraminidase(NA)gene of Influenza virus A/FM,1/47 was amplified by RT—PCR and cloned into adenovirus vector pTrack—CM V The recombinan t plasmid an d adenovirus DNA Was cotransformated into E coli BJ5 1 83 an d the recombinan t adenovirus genomic DNA Was obtained through homological recombination.The recombinan t adenovirus was gained after tran sfecting 293 cell line with the genomic DNA.The intergration of NA in resultan t adenovirus an d the expression of NA gene were confilrned by PCR an d western—blot respectively.BALB/c mice were immunized intranasaUy and orally respectively.ELISA result showed that systemi c immue responses to Influenza virus could be induced efectively in both intran asal an d oral route.Th e immunization efficacy of intran asal route Was superior to that of oral route.