Citation: ZHAO Xiao, HAN Feng, WANG Yan, YAN G, YING Ge, LING Yong, WAN G, WU Shu. Construction of Recombinant Adenovirus Expressing Influenza virus Neuraminidase Gene .VIROLOGICA SINICA, 2003, 18(3) : 213-216.

Construction of Recombinant Adenovirus Expressing Influenza virus Neuraminidase Gene

  • Available online: 18 June 2003
  • Abstract:1]he neuraminidase(NA)gene of Influenza virus A/FM,1/47 was amplified by RT—PCR and cloned into adenovirus vector pTrack—CM V The recombinan t plasmid an d adenovirus DNA Was cotransformated into E coli BJ5 1 83 an d the recombinan t adenovirus genomic DNA Was obtained through homological recombination.The recombinan t adenovirus was gained after tran sfecting 293 cell line with the genomic DNA.The intergration of NA in resultan t adenovirus an d the expression of NA gene were confilrned by PCR an d western—blot respectively.BALB/c mice were immunized intranasaUy and orally respectively.ELISA result showed that systemi c immue responses to Influenza virus could be induced efectively in both intran asal an d oral route.Th e immunization efficacy of intran asal route Was superior to that of oral route.

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    Construction of Recombinant Adenovirus Expressing Influenza virus Neuraminidase Gene

    • 1. 1.Key Laboratory of Molecular Virology and Genetic Engineering,National Institute f0r Viral Disease Prevetion and Control,ChinaCDC,Beifing 100052,China

    Abstract: Abstract:1]he neuraminidase(NA)gene of Influenza virus A/FM,1/47 was amplified by RT—PCR and cloned into adenovirus vector pTrack—CM V The recombinan t plasmid an d adenovirus DNA Was cotransformated into E coli BJ5 1 83 an d the recombinan t adenovirus genomic DNA Was obtained through homological recombination.The recombinan t adenovirus was gained after tran sfecting 293 cell line with the genomic DNA.The intergration of NA in resultan t adenovirus an d the expression of NA gene were confilrned by PCR an d western—blot respectively.BALB/c mice were immunized intranasaUy and orally respectively.ELISA result showed that systemi c immue responses to Influenza virus could be induced efectively in both intran asal an d oral route.Th e immunization efficacy of intran asal route Was superior to that of oral route.

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