Citation: ZHAO Shu—ling, LIANG Chang—yong, HONG Jing-jun, PENG Hui—yin*. cDNA Clone and Sequence Analysis of Segment 4 of Dendrolimus punctatus cytoplasmic polyhedrosis virus .VIROLOGICA SINICA, 2003, 18(3) : 246-250.

cDNA Clone and Sequence Analysis of Segment 4 of Dendrolimus punctatus cytoplasmic polyhedrosis virus

  • Available online: 18 June 2003
  • Abstract:Dendrolimus punctatus cytoplasmic polyhedrosis virus(DpCPV)genome dsRNA wag extracted an d S4 was separated.RT—PCR amplification technique Wag adopted to clone the viral genomic dsRNA S4 flor sequence determination.The results showed:the full length of S4 is 3262bp,it contains one large open reading fram e an d encodes a polype ptide with 1058 amino acids in length.Tlle similarity of predicted protein were 94% 、92% an d 22% with LdCPV、BmCPV an d RRSV respectively. Amino acid sequences simi lar to a GTPase、methyltran sferase of Methanosarcina mazef Goel motifs were also present in the deduced protein.

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    cDNA Clone and Sequence Analysis of Segment 4 of Dendrolimus punctatus cytoplasmic polyhedrosis virus

    • 1. Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan430071,China

    Abstract: Abstract:Dendrolimus punctatus cytoplasmic polyhedrosis virus(DpCPV)genome dsRNA wag extracted an d S4 was separated.RT—PCR amplification technique Wag adopted to clone the viral genomic dsRNA S4 flor sequence determination.The results showed:the full length of S4 is 3262bp,it contains one large open reading fram e an d encodes a polype ptide with 1058 amino acids in length.Tlle similarity of predicted protein were 94% 、92% an d 22% with LdCPV、BmCPV an d RRSV respectively. Amino acid sequences simi lar to a GTPase、methyltran sferase of Methanosarcina mazef Goel motifs were also present in the deduced protein.

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