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Volume 18 Issue 3
June 2003
    Citation: LIU Sheng—wang, DU En—qi, KONG Xian —gan g, YANG Zeng—qi. The Molecular Characteristics of mRNAs and mRNA6 cDNA of lnfectious bronchitis virus LX4 Isolated in China .VIROLOGICA SINICA, 2003, 18(3) : 265-270.
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    The Molecular Characteristics of mRNAs and mRNA6 cDNA of lnfectious bronchitis virus LX4 Isolated in China

    • 1.

      National Laboratory of Veterinary Biotechnology,Institute ofHarbin Veterinary Medicine,CAAS,Haerbin 150001

    • 2.

      College of Animal Science．Northwest Sci-Tech University of Agriculture and Forestry,Yangling 712100,China

    • Available online: 18 June 2003
    • According to the published IBV gene sequence, a pair of specific primers were designed and synthesized. A fragment of 1.7kb including mRNAs and mRNA_6 cDNA of infectious bronchitis virus LX4 was amplified, cloned and sequenced. Then the nucleotide and the deduced amino acid sequence of this fragment were analyzed. The results showed that N gene of IBV LX4 sharedthe highest identity with SD/97/02 and HB that were both isolated in China. However, the identity is different in three conservative basic regions of N protein. The deduced amino acid sequence of LX4 shared the highest identity with M41,Beau, H52 and HB in position 66-88, with SD/97/02 strain in position 181-234 and with H120 and ZJ971 in position 334-373 respectively. Meanwhile, we found that LX4 and H120 showed completely identical in position 350-409 both in corresponding nucleotide and deduced amino acid. All these data above showed that mRNA_5 and mRNA_6 cDNA of IBV LX4 shared more characteristics with other isolates in China than the aboard. In a
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      The Molecular Characteristics of mRNAs and mRNA6 cDNA of lnfectious bronchitis virus LX4 Isolated in China

      • 1. National Laboratory of Veterinary Biotechnology,Institute ofHarbin Veterinary Medicine,CAAS,Haerbin 150001
      • 2. College of Animal Science．Northwest Sci-Tech University of Agriculture and Forestry,Yangling 712100,China

      Abstract: According to the published IBV gene sequence, a pair of specific primers were designed and synthesized. A fragment of 1.7kb including mRNAs and mRNA_6 cDNA of infectious bronchitis virus LX4 was amplified, cloned and sequenced. Then the nucleotide and the deduced amino acid sequence of this fragment were analyzed. The results showed that N gene of IBV LX4 sharedthe highest identity with SD/97/02 and HB that were both isolated in China. However, the identity is different in three conservative basic regions of N protein. The deduced amino acid sequence of LX4 shared the highest identity with M41,Beau, H52 and HB in position 66-88, with SD/97/02 strain in position 181-234 and with H120 and ZJ971 in position 334-373 respectively. Meanwhile, we found that LX4 and H120 showed completely identical in position 350-409 both in corresponding nucleotide and deduced amino acid. All these data above showed that mRNA_5 and mRNA_6 cDNA of IBV LX4 shared more characteristics with other isolates in China than the aboard. In a

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