A Research of The Stability Based on A Cell M odel Persistently Infected By A Field．isolated Virulent Strain of CSFV
Abstract: We jointly applied immunofluorescence technique, RT-PCR assay and flow cytometer to evaluate the stability of a cell model persistently infected by Classical swine fever virus (CSFV39), which was established by our laboratory and named CSFV39-PK15. The experiment results indicated that this cell model was quite stable. Even in the 128th CSFV39-PK15 passage cells, we still could detect the existence of CSFV39. Both immunofluorescence antibody test and RT-PCR assay showed positive results. We also found there was no significant difference in their cell cycle between normal control PK-15 and CSFV39-PK15. Sequence homology analysis of the same fragment revealed that CSFV39 had the highest homology compared with Shimen strain, amounting to 99.02%.