Citation: DING Qiao, LI Jian-jun, CHEN Pu. Expression and Genic Imm unization of Glycoprotein gB Gene ofM arek’s disease virus .VIROLOGICA SINICA, 2003, 18(3) : 275-278.

Expression and Genic Imm unization of Glycoprotein gB Gene ofM arek’s disease virus

  • Available online: 18 June 2003
  • gB gene of Marek’s disease virus (MDV) was inserted into pcDNA3, forming eukaryotic expression recombinant plasmid pcDNA3-gB. Recombinant plasmid pcDNA3-gB was identified by BamHⅠI and PCR. PcDNA3-gB was transfected into COS-7 cells in vitro. Expressed protein was detected by indirect immunofluorescence assay. Immune test groups were inoculated with pcDNA3-gB intramuscularly. Two weeks later, test groups were boosted with the same pcDNA3-gB. Since then, two weeks later chickens were challenged with vMDV. Immune effects showed that the immune protection rate of test group reached to 72.2%. The results proved that pcDNA3-gB DNA immunization elicited immune protection.

  • 加载中
  • 加载中

Article Metrics

Article views(4456) PDF downloads(865) Cited by()

Related
Proportional views

    Expression and Genic Imm unization of Glycoprotein gB Gene ofM arek’s disease virus

    • 1. Key Laboratory of Animal Diseases Diagnostic and Immunology of Agriculture Ministry of China,Nanjing Agricultural University,Nanjing 210095.China
    • 2. Department of Veterinary medicine,Foshan Science&Technology College,Nanhai 528231,Chinaf1.Key Laboratory of Animal Diseases Diagnostic and Immunology of Agriculture Ministry of China,Nanjing Agricultural University,Nanjing 210095.China

    Abstract: gB gene of Marek’s disease virus (MDV) was inserted into pcDNA3, forming eukaryotic expression recombinant plasmid pcDNA3-gB. Recombinant plasmid pcDNA3-gB was identified by BamHⅠI and PCR. PcDNA3-gB was transfected into COS-7 cells in vitro. Expressed protein was detected by indirect immunofluorescence assay. Immune test groups were inoculated with pcDNA3-gB intramuscularly. Two weeks later, test groups were boosted with the same pcDNA3-gB. Since then, two weeks later chickens were challenged with vMDV. Immune effects showed that the immune protection rate of test group reached to 72.2%. The results proved that pcDNA3-gB DNA immunization elicited immune protection.

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return