DING Qiao, LI Jian-jun, CHEN Pu.Expression and Genic Imm unization of Glycoprotein gB Gene ofM arek’s disease virus .VIROLOGICA SINICA, 2003, 18(3): 275-278.
Citation:
DING Qiao, LI Jian-jun, CHEN Pu.
Expression and Genic Imm unization of Glycoprotein gB Gene ofM arek’s disease virus .VIROLOGICA SINICA, 2003, 18(3)
: 275-278.
Expression and Genic Imm unization of Glycoprotein gB Gene ofM arek’s disease virus
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1.
Key Laboratory of Animal Diseases Diagnostic and Immunology of Agriculture Ministry of China,Nanjing Agricultural University,Nanjing 210095.China
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2.
Department of Veterinary medicine,Foshan Science&Technology College,Nanhai 528231,Chinaf1.Key Laboratory of Animal Diseases Diagnostic and Immunology of Agriculture Ministry of China,Nanjing Agricultural University,Nanjing 210095.China
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Available online:
18 June 2003
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Abstract
gB gene of Marek’s disease virus (MDV) was inserted into pcDNA3, forming eukaryotic expression recombinant plasmid pcDNA3-gB. Recombinant plasmid pcDNA3-gB was identified by BamHⅠI and PCR. PcDNA3-gB was transfected into COS-7 cells in vitro. Expressed protein was detected by indirect immunofluorescence assay. Immune test groups were inoculated with pcDNA3-gB intramuscularly. Two weeks later, test groups were boosted with the same pcDNA3-gB. Since then, two weeks later chickens were challenged with vMDV. Immune effects showed that the immune protection rate of test group reached to 72.2%. The results proved that pcDNA3-gB DNA immunization elicited immune protection.
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References
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Proportional views
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