Examination ofSpherical Viruses from Mytilus edulis

Abstract：A lot of spherical virions were observed in internal organiT．ation such as gill and mantellum of the diseased Mytilus edulis with negative staining method．The diameter of V on is around 8()_一 200nm with dual unit membran e．Sam e virions were observed in connective tissuese of infected organ s with ultrathin section electronmicroscopic observ ation．Th e“occlusion body’’were observed in cell matrix an d no inclusion bod y was present．A few same virions were observed in internal organization of normal Ruditapes variega~，Neptunea cumingi an d Dstrea lalienwhanensis with negative staining method．，nle normal Chlorostoma rusticum Can be infected by organizational liquid with the viruses from diseased Mytilus edulis through mouth and injured section on skin．Th e virus was purified from the diseased tissue by sucrose gradient centrifugation with 25 0oo r／min．3h．Th e virus sediment lies between 2O％ ～ 3O％ of sucrose． Key word：Mytilus edulis；SphericalAbstract：A lot of spherical virions were observed in internal organiT．ation such as gill and mantellum of the diseased Mytilus edulis with negative staining method．The diameter of V on is around 8()_一 200nm with dual unit membran e．Sam e virions were observed in connective tissuese of infected organ s with ultrathin section electronmicroscopic observ ation．Th e“occlusion body’’were observed in cell matrix an d no inclusion bod y was present．A few same virions were observed in internal organization of normal Ruditapes variega~，Neptunea cumingi an d Dstrea lalienwhanensis with negative staining method．，nle normal Chlorostoma rusticum Can be infected by organizational liquid with the viruses from diseased Mytilus edulis through mouth and injured section on skin．Th e virus was purified from the diseased tissue by sucrose gradient centrifugation with 25 0oo r／min．3h．Th e virus sediment lies between 2O％ ～ 3O％ of sucrose．