Citation: ZUO Ze—hua.LI Hui.WU Xin—xing, . Expression of Recom binant pEGFP·HPV16 E7 in Eukaryocyte .VIROLOGICA SINICA, 2003, 18(4) : 344-347.

Expression of Recom binant pEGFP·HPV16 E7 in Eukaryocyte

  • Available online: 05 August 2003
  • Abstract:In order to make bases for construction of animal tumor model which expresses EGFP— HPV l 6E7,recombinant expression plasmid was constructed by techniques of gene recombination after screening an d identifing by restriction and PCR.Then the recombinant was tran sfected into mouse liver cancer cell by techniques of gene transfection and detected expression by fluoroscopy.pEGFP—HPV 1 6 E7 was identified by enzyme digestion an d PCR.Th e resent showed that the length .inserted location and direction of the target gene was correct an d the expression of EGFP in tran sfected cell was observed Because of it’S pEGFP—HPV 1 6E7 expression plasmid that mak es it easy to assess the expression of EGFP-HPV 1 6E7 fusion protein and to sift the transfected cells.

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    Expression of Recom binant pEGFP·HPV16 E7 in Eukaryocyte

    • 1. Virus Research Institution ofMedical College,Wuhan University Wuhan 430071,China

    Abstract: Abstract:In order to make bases for construction of animal tumor model which expresses EGFP— HPV l 6E7,recombinant expression plasmid was constructed by techniques of gene recombination after screening an d identifing by restriction and PCR.Then the recombinant was tran sfected into mouse liver cancer cell by techniques of gene transfection and detected expression by fluoroscopy.pEGFP—HPV 1 6 E7 was identified by enzyme digestion an d PCR.Th e resent showed that the length .inserted location and direction of the target gene was correct an d the expression of EGFP in tran sfected cell was observed Because of it’S pEGFP—HPV 1 6E7 expression plasmid that mak es it easy to assess the expression of EGFP-HPV 1 6E7 fusion protein and to sift the transfected cells.

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