Expression of Recom binant pEGFP·HPV16 E7 in Eukaryocyte

Abstract：In order to make bases for construction of animal tumor model which expresses EGFP— HPV l 6E7，recombinant expression plasmid was constructed by techniques of gene recombination after screening an d identifing by restriction and PCR．Then the recombinant was tran sfected into mouse liver cancer cell by techniques of gene transfection and detected expression by fluoroscopy．pEGFP—HPV 1 6 E7 was identified by enzyme digestion an d PCR．Th e resent showed that the length ．inserted location and direction of the target gene was correct an d the expression of EGFP in tran sfected cell was observed Because of it’S pEGFP—HPV 1 6E7 expression plasmid that mak es it easy to assess the expression of EGFP-HPV 1 6E7 fusion protein and to sift the transfected cells．