Construction of Eukarytic Expression Vector Containing PCV-2 ORF2 and Its Expression

According to the published OI 2 gene sequence of PCV一2．two primers were designed to amplify the ORF2 gene from PK一1 5 cells inoculated by PCV一2．It was cloned into the pSecTag2 vector,and the recombinant plasmid named pSecORF2 was obtained．Another primer was designed to amplify OI 2 gene including signal peptide sequence from pSecORF2．Then it was cloned into pIREShyg vector．Th e recombinan t expression vector plRESiORF2 was constructed．CHO cells were tran sfected in vitro with them． Th e expression of ORF2 gene was detected by indirect immunofluorescence assay(If1A、．Further research iS underway to study the biological activity of OI protein an d to establish the diagnostic kit of PCV