Sequencing and Analysis on the F and HN Genes of Avian paramyxovirus Type I Isolated from Parrot

Virion RNA was abstracted from purified type I Avian paramyxovirus strain YN—PA0 1 (isolated from parrot)and used as a template．The fragment containing the fusion gene(F)and hemagglutinin-·neuraminidase gene(HN)of the isolate was amplified by RT-·PCR and cloned to the pMD 1 8一T Vector．Using primer walking method the complete sequence of F-HN genes was obtained finally． And the respective amino acid sequence was deduced．Through relative software the phylogenetic trees on F gene an d HN gene were constructed between strain YN—PA0 1 an d reference strains．The results showed that strain YN—PA01 comparing with reference strains displays 98．7％ -83．2％ nucleotide homology an d 98．1％-86．2％ amino acid homology on F gene；97．4％-79．1％ nucleotide homology an d 97．2％ -83．2％ amino acid homology on HN gene．Additional 6 amino acids are encoded by the HN gene ORF of strain YN—PA01 comparing with national reference strains．Th e studied strain YN—PA01 exhibits highest identities with strain JS／5／0 1／Go either an alyzed on F gene or HN gene．