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Volume 18 Issue 5
October 2003
    Citation: WANG Ping—zhong, BAI Xue—Fan, PAN Lei, ZHANG Ying, HUANG Chang—xing. Expression of Hantaan virus S Gene in B Lymphoblastoid Cell Lines from Patients with Hemorrhagic Fever with Renal Syndrome .VIROLOGICA SINICA, 2003, 18(5) : 420-422.
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    Expression of Hantaan virus S Gene in B Lymphoblastoid Cell Lines from Patients with Hemorrhagic Fever with Renal Syndrome

    • 1.

      The Center of Diagnosis and Treatmentfor Infectious Diseases of Chinese PLA，Tangdu Hospital,Fourth Military Medical University,Xi’an 710038．China

    • Available online: 05 October 2003
    • Abstract：In order to construct the eukaryotic expression vector of月“man immunodeficieacy virus 1 (HIV-I)core protein gene(pCI—neoGAG)，gag gene was acquired from the plasmid pKSGAG by restriction endonuclease digestion (Xba I ISal I)and ligated onto the eukaryotic expression vector pCI．neo．The construction of pCI．neoGAG was confirmed by restriction endonuclease digestion(Xba I ISal I)analysis and DNA sequencing．Th en the pCI-neoGAG was transfected into p8 1 5 cells by lipofectine and the expressed product was detected by indirect immunofluorescence after G4 1 8 selection for 4 weeks．The results showed that the core protein was expressed in p8 1 5 cells successfully．which will be used for the further test of HIV．1 DNA vaccine．
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      Expression of Hantaan virus S Gene in B Lymphoblastoid Cell Lines from Patients with Hemorrhagic Fever with Renal Syndrome

      • 1. The Center of Diagnosis and Treatmentfor Infectious Diseases of Chinese PLA，Tangdu Hospital,Fourth Military Medical University,Xi’an 710038．China

      Abstract: Abstract：In order to construct the eukaryotic expression vector of月“man immunodeficieacy virus 1 (HIV-I)core protein gene(pCI—neoGAG)，gag gene was acquired from the plasmid pKSGAG by restriction endonuclease digestion (Xba I ISal I)and ligated onto the eukaryotic expression vector pCI．neo．The construction of pCI．neoGAG was confirmed by restriction endonuclease digestion(Xba I ISal I)analysis and DNA sequencing．Th en the pCI-neoGAG was transfected into p8 1 5 cells by lipofectine and the expressed product was detected by indirect immunofluorescence after G4 1 8 selection for 4 weeks．The results showed that the core protein was expressed in p8 1 5 cells successfully．which will be used for the further test of HIV．1 DNA vaccine．

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