Citation: LI Guang—yu, HUANG Chang—xing, PAN Lei, MOU Dan —lei, LI Xing—hong, ZAN G Yin, YANG Wei—song, BAI Xue—fan. Construction of Prokaryotic Expression Vectors Harboring HV S and S Partial Gene Segments and Identification of Expressed Nucleocapsid Protein .VIROLOGICA SINICA, 2003, 18(5) : 428-431.

Construction of Prokaryotic Expression Vectors Harboring HV S and S Partial Gene Segments and Identification of Expressed Nucleocapsid Protein

  • Available online: 05 October 2003
  • Abstract:To study the antigenicity of prokaryotic expressed products of Hantavirus(HV)S partial gene(502—1326bp)an d obtain data for serotyping antigen,the prokaryotic expression vectors pRSET A—S,pRSET A—S—C were constructed respectively.The recombinant expression plasmids were fans— formed into BL2 I(DE3)pLySs.Th e expression product were analyzed by SDS—PAGE and Western—blot. Th e results showed that the proteins were expressed at high level respectively an d could be recognized by patients’(infected by HTNV)sera distinctly and peculiarly.Th e truncated NP(aa155 to 429)could be considered as serotyping an tigen.

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    Construction of Prokaryotic Expression Vectors Harboring HV S and S Partial Gene Segments and Identification of Expressed Nucleocapsid Protein

    • 1. Department ofInfectious Diseases,Tangdu Hospital,Fourth Military Medical University,X an 710038,China

    Abstract: Abstract:To study the antigenicity of prokaryotic expressed products of Hantavirus(HV)S partial gene(502—1326bp)an d obtain data for serotyping antigen,the prokaryotic expression vectors pRSET A—S,pRSET A—S—C were constructed respectively.The recombinant expression plasmids were fans— formed into BL2 I(DE3)pLySs.Th e expression product were analyzed by SDS—PAGE and Western—blot. Th e results showed that the proteins were expressed at high level respectively an d could be recognized by patients’(infected by HTNV)sera distinctly and peculiarly.Th e truncated NP(aa155 to 429)could be considered as serotyping an tigen.

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