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Volume 18 Issue 5
October 2003
    Citation: LI Guang—yu, HUANG Chang—xing, PAN Lei, MOU Dan —lei, LI Xing—hong, ZAN G Yin, YANG Wei—song, BAI Xue—fan. Construction of Prokaryotic Expression Vectors Harboring HV S and S Partial Gene Segments and Identification of Expressed Nucleocapsid Protein .VIROLOGICA SINICA, 2003, 18(5) : 428-431.
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    Construction of Prokaryotic Expression Vectors Harboring HV S and S Partial Gene Segments and Identification of Expressed Nucleocapsid Protein

    • 1.

      Department ofInfectious Diseases，Tangdu Hospital,Fourth Military Medical University,X an 710038，China

    • Available online: 05 October 2003
    • Abstract：To study the antigenicity of prokaryotic expressed products of Hantavirus(HV)S partial gene(502—1326bp)an d obtain data for serotyping antigen，the prokaryotic expression vectors pRSET A—S，pRSET A—S—C were constructed respectively．The recombinant expression plasmids were fans— formed into BL2 I(DE3)pLySs．Th e expression product were analyzed by SDS—PAGE and Western—blot． Th e results showed that the proteins were expressed at high level respectively an d could be recognized by patients’(infected by HTNV)sera distinctly and peculiarly．Th e truncated NP(aa155 to 429)could be considered as serotyping an tigen．
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      Construction of Prokaryotic Expression Vectors Harboring HV S and S Partial Gene Segments and Identification of Expressed Nucleocapsid Protein

      • 1. Department ofInfectious Diseases，Tangdu Hospital,Fourth Military Medical University,X an 710038，China

      Abstract: Abstract：To study the antigenicity of prokaryotic expressed products of Hantavirus(HV)S partial gene(502—1326bp)an d obtain data for serotyping antigen，the prokaryotic expression vectors pRSET A—S，pRSET A—S—C were constructed respectively．The recombinant expression plasmids were fans— formed into BL2 I(DE3)pLySs．Th e expression product were analyzed by SDS—PAGE and Western—blot． Th e results showed that the proteins were expressed at high level respectively an d could be recognized by patients’(infected by HTNV)sera distinctly and peculiarly．Th e truncated NP(aa155 to 429)could be considered as serotyping an tigen．

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