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Volume 18 Issue 5
October 2003
    Citation: GU Chao-jiang, ZHENG Cong—yi, WU Ji—bin, QU San—fu, YANG Wei, CHANG Hui—yun. Rapid Selection of the Cells Persistently Infected with Foot-and-mouth disease virus and the Study of Natures in Persistent Infection Cells .VIROLOGICA SINICA, 2003, 18(5) : 459-463.
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    Rapid Selection of the Cells Persistently Infected with Foot-and-mouth disease virus and the Study of Natures in Persistent Infection Cells

    • 1.

      College of Life Sciences，Wuhan University，430072，China

    • Available online: 05 October 2003
    • Abstract：A baby hamster kidney cell line(BHK一21) infected by foot—and-mouth disease virus (OGBF1 5 strain)was treated with NH4Cl，then the cells survived were selected and cloned．32 positive—cloned cell strains were obtained．One strain fBHK—ROp)was randomly selected and subcul— tured as usual and an alysed with RT_PCR．transmission electron microscope an d flow cytometry．The results demonstrated that the 4th，1 6th，36th generation BHK—ROp cell still harboured ⅥDV which had no chan ges on the growth features of the cells．They had the radical characteristics of viral persistent infection．As noted above，the NH4Cl weak base method used for establishment of the cell line persistently infected with virus was particularly effective．
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      Rapid Selection of the Cells Persistently Infected with Foot-and-mouth disease virus and the Study of Natures in Persistent Infection Cells

      • 1. College of Life Sciences，Wuhan University，430072，China

      Abstract: Abstract：A baby hamster kidney cell line(BHK一21) infected by foot—and-mouth disease virus (OGBF1 5 strain)was treated with NH4Cl，then the cells survived were selected and cloned．32 positive—cloned cell strains were obtained．One strain fBHK—ROp)was randomly selected and subcul— tured as usual and an alysed with RT_PCR．transmission electron microscope an d flow cytometry．The results demonstrated that the 4th，1 6th，36th generation BHK—ROp cell still harboured ⅥDV which had no chan ges on the growth features of the cells．They had the radical characteristics of viral persistent infection．As noted above，the NH4Cl weak base method used for establishment of the cell line persistently infected with virus was particularly effective．

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