Partial Characteristic Comparison Between Two Aquareovirus Isolates

Aquareoviruses，which belong to a newly identified genus of the family Reoviridae，are agents infecting all kind of aquatic animals．Grass carp reovirus(GCRV)was isolated from outbreak grass carp fingerling in South China freshwater region，and Threadfin reovirus(1f1W )is a Singapore isolate from acute diseased marine threadfin fingerling．In this study,the partial characterization between GCRV and TFV were compared．It showed that two viruses Can cause CPE in CIK cell line，but not all the same in the other compared fish cell lines．In addition．their RNA genome compo sitions ale diferent by both electrophoresis and RT_PCR．It is interesting to point out that．based on serological related Westem blot analysis．me antiserum against GCRV could cross detecting some of the proteins found in TFV but not all of the proteins．It suggested they may share some similar epitopes．